The crystal structure of manganese peroxidase from Phanerochaete chrysosporium at 2.06-A resolution.

The nop gene from Phanerochaete chrysosporium encodes a peroxidase with novel structural features.

Inspection of the genome of the ligninolytic basidiomycete Phanerochaete chrysosporium revealed an unusual peroxidase_like sequence. The corresponding full length cDNA was sequenced and an archetypal secretion signal predicted. The deduced mature protein (NoP, novel peroxidase) contains 295 aa residues and is therefore considerably shorter than other Class II (fungal) peroxidases, such as ligni...

Lipid Peroxidation by the Manganese Peroxidase of Phanerochaete chrysosporium Is the Basis for Phenanthrene Oxidation

The manganese peroxidase (MnP) of Phanerochaete chrysosporium supported Mn(II)-dependent, H202independent lipid peroxidation, as shown by two findings: linolenic acid was peroxidized to give products that reacted with thiobarbituric acid, and linoleic acid was peroxidized to give hexanal. MnP also supported the slow oxidation of phenanthrene to 2,2'-diphenic acid in a reaction that required Mn(...

Heterologous Expression of Phanerochaete chrysoporium Glyoxal Oxidase and its Application for the Coupled Reaction with Manganese Peroxidase to Decolorize Malachite Green

cDNA of the glx1 gene encoding glyoxal oxidase (GLX) from Phanerochaete chrysosporium was isolated and expressed in Pichia pastoris. The recombinant GLX (rGLX) produces H(2)O(2) over 7.0 nmol/min/mL using methyl glyoxal as a substrate. Use of rGLX as a generator of H(2)O(2) improved the coupled reaction with recombinant manganese peroxidase resulting in decolorization of malachite green up to 1...

Phenanthrene Removal from Soil by a Strain of Aspergillus niger Producing Manganese Peroxidase of Phanerochaete chrysosporium

Degradation of Phenol Derivatives by a Phanerochaete Chrysosporium Strain

The white rot fungus Phanerochaete chrysosporium has significant pollutant-degrading capabilities with its oxidoreductases. The lignin-degrading enzyme system of this fungus also allows the breakdown of different organic pollutants (xenobiotics). The most significant enzymes in these processes are the lignin peroxidase and the manganese peroxidase. In this study, Phanerochaete chrysosporium str...